View source for Polony DNA sequencing. 2006 Curr Protoc Mol Biol.


<span style="font-size:16px;">Polony DNA sequencing provides an inexpensive, accurate, high-throughput way to resequence genomes of interest by comparison to a reference genome. Mate-paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR. These serve as templates for sequencing by fluorescent nonamer ligation reactions on a microscope slide. Each sequencing run results in millions of 26-bp reads that can be aligned to the reference genome, allowing the identification of differences between sequences.<br/> <br/> [https://currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1002/0471142727.mb0708s76 Link to full article]<br/> <br/> <br/> Porreca GJ, Shendure J, Church GM. Harvard Medical School, Boston, Massachusetts, USA.<br/> <br/> <span title="Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.].">Curr Protoc Mol Biol.</span>&nbsp;2006.11.</span>

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