Difference between revisions of "Polony DNA sequencing. 2006 Curr Protoc Mol Biol."
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| − | + | <span style="font-size:16px;">Polony DNA sequencing provides an inexpensive, accurate, high-throughput way to resequence genomes of interest by comparison to a reference genome. Mate-paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR. These serve as templates for sequencing by fluorescent nonamer ligation reactions on a microscope slide. Each sequencing run results in millions of 26-bp reads that can be aligned to the reference genome, allowing the identification of differences between sequences.<br/> <br/> [https://currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1002/0471142727.mb0708s76 Link to full article]<br/> <br/> <br/> Porreca GJ, Shendure J, Church GM. Harvard Medical School, Boston, Massachusetts, USA.<br/> <br/> <span title="Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.].">Curr Protoc Mol Biol.</span> 2006.11.</span> | |
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Latest revision as of 17:43, 2 July 2020
Polony DNA sequencing provides an inexpensive, accurate, high-throughput way to resequence genomes of interest by comparison to a reference genome. Mate-paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR. These serve as templates for sequencing by fluorescent nonamer ligation reactions on a microscope slide. Each sequencing run results in millions of 26-bp reads that can be aligned to the reference genome, allowing the identification of differences between sequences.
Link to full article
Porreca GJ, Shendure J, Church GM. Harvard Medical School, Boston, Massachusetts, USA.
Curr Protoc Mol Biol. 2006.11.
