Difference between revisions of "GDNA Shipment process from abroad"
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− | <p>Please download and refer to the following documents for information on gDNA methods of preparation and delivery.</p> | + | <p><span style="font-size: large"><b><span id="Informaion_of_Sample.28gDNA.29_.C2.A0delivery_preparation" class="mw-headline">Informaion for Sample(gDNA) Preparation Delivery </span></b></span></p> |
− | <p>1. <a href="/userfiles/DNA_sample_sheet.doc">Sample Shipping Information </ | + | <p><span style="font-size: small">1) Method of gDNA preparation for sending out.</span></p> |
− | < | + | <ul> |
+ | <li><span style="font-size: small"> DNA should be dissolved in TE buffer (Tris 10mM pH 8,0, 0.1 mM EDTA) or 10 mM Tris pH 8.0. Do Not Dissolve in Water. </span></li> | ||
+ | <li><span style="font-size: small"> DNA requirement : total 50-100ug of gDNA with >50 ng/ul </span></li> | ||
+ | <li><span style="font-size: small"> Sample purity: OD260/280=1.8~2.0; free of protein, RNA, or other visible contamination </span></li> | ||
+ | <li><span style="font-size: small"> The sender must provide at least one information among “Sample QC data” and “Gel image of DNA electrophoresis” </span></li> | ||
+ | </ul> | ||
+ | <p><span style="font-size: small">2) Sender information</span></p> | ||
+ | <ul> | ||
+ | <li><span style="font-size: small"> Name : </span></li> | ||
+ | <li><span style="font-size: small"> Affiliation: </span></li> | ||
+ | <li><span style="font-size: small"> Address: </span></li> | ||
+ | <li><span style="font-size: small"> Telephone: </span></li> | ||
+ | <li><span style="font-size: small"> Email: </span></li> | ||
+ | </ul> | ||
+ | <p><span style="font-size: small"> 3) Sample information</span></p> | ||
+ | <ul> | ||
+ | <li><span style="font-size: small"><span style="font-family: Arial"><span style="line-height: 150%; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: KO" lang="EN-US"><span style="mso-list: Ignore"><span style="font: 7pt 'Times New Roman'"> </span></span></span><span style="line-height: 150%; mso-fareast-font-family: '맑은 고딕'; mso-fareast-language: KO" lang="EN-US">Sample type: DNA</span></span></span></li> | ||
+ | <li><span style="font-size: small"><span style="font-family: Arial"><span style="line-height: 150%; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: KO" lang="EN-US"><span style="mso-list: Ignore"><span style="font: 7pt 'Times New Roman'"> </span></span></span><span style="line-height: 150%; mso-fareast-font-family: '맑은 고딕'; mso-fareast-language: KO" lang="EN-US">Condition: <u>DNA in TE buffer or EB buffer or equivalent. Not in water</u></span> </span></span></li> | ||
+ | <li><span style="font-size: small"><span style="font-family: Arial"><span style="line-height: 150%; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: KO" lang="EN-US"><span style="mso-list: Ignore"><span style="font: 7pt 'Times New Roman'"> </span></span></span><span style="line-height: 150%; mso-fareast-font-family: '맑은 고딕'; mso-fareast-language: KO" lang="EN-US">Sample QC data (</span><span style="line-height: 150%; color: red" lang="EN-US">*</span><span style="line-height: 150%; color: red; mso-fareast-font-family: '맑은 고딕'; mso-fareast-language: KO" lang="EN-US"> </span><span style="line-height: 150%; color: black; mso-fareast-font-family: '맑은 고딕'; mso-fareast-language: KO" lang="EN-US">is Essential</span><span style="line-height: 150%; mso-fareast-font-family: '맑은 고딕'; mso-fareast-language: KO" lang="EN-US">)</span></span></span></li> | ||
+ | <li><span style="font-size: small"><span style="font-family: Arial"><span style="line-height: 150%; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: KO" lang="EN-US"><span style="mso-list: Ignore"><span style="font: 7pt 'Times New Roman'"> </span></span></span><span style="line-height: 150%; mso-fareast-font-family: '맑은 고딕'; mso-fareast-language: KO" lang="EN-US">Gel image of DNA electrophoresis</span></span> </span></li> | ||
+ | </ul> | ||
+ | <p><span style="font-size: small">4) DNA packing information This way is only available when DNA is dissolved in TE or Tris buffer (Please read Note).<br /> | ||
+ | If not, please pack DNA with icepack in icebox and use Fedex or DHL service.</span></p> | ||
+ | <ul> | ||
+ | <li><span style="font-size: small">Mark sample information on tube (concentration, sample name…what you want) </span></li> | ||
+ | <li><span style="font-size: small">Seal the cap of tube with parafilm well </span></li> | ||
+ | <li><span style="font-size: small">Wrap the tube with paper towel or equivalent </span></li> | ||
+ | <li><span style="font-size: small">Put into the 50 ml Falcon tube and close the cap </span></li> | ||
+ | <li><span style="font-size: small">Send the sample with air cushion envelop by FEDEX or DHL or equivalent. </span></li> | ||
+ | </ul> | ||
+ | <p><span style="font-size: small">Please download and refer to the following documents for information on gDNA methods of preparation and delivery.</span></p> | ||
+ | <p> </p> | ||
+ | <hr /> | ||
+ | <p><span style="font-size: small">1. </span><a href="/userfiles/DNA_sample_sheet.doc"><span style="font-size: small">Sample Shipping Information </span></a><span style="font-size: small">2. </span><a href="/userfiles/Declaration_of_Shipment_for_anyone.doc"><span style="font-size: small">Declaration </span></a></p> |
Latest revision as of 11:32, 25 November 2011
Informaion for Sample(gDNA) Preparation Delivery
1) Method of gDNA preparation for sending out.
- DNA should be dissolved in TE buffer (Tris 10mM pH 8,0, 0.1 mM EDTA) or 10 mM Tris pH 8.0. Do Not Dissolve in Water.
- DNA requirement : total 50-100ug of gDNA with >50 ng/ul
- Sample purity: OD260/280=1.8~2.0; free of protein, RNA, or other visible contamination
- The sender must provide at least one information among “Sample QC data” and “Gel image of DNA electrophoresis”
2) Sender information
- Name :
- Affiliation:
- Address:
- Telephone:
- Email:
3) Sample information
- Sample type: DNA
- Condition: DNA in TE buffer or EB buffer or equivalent. Not in water
- Sample QC data (* is Essential)
- Gel image of DNA electrophoresis
4) DNA packing information This way is only available when DNA is dissolved in TE or Tris buffer (Please read Note).
If not, please pack DNA with icepack in icebox and use Fedex or DHL service.
- Mark sample information on tube (concentration, sample name…what you want)
- Seal the cap of tube with parafilm well
- Wrap the tube with paper towel or equivalent
- Put into the 50 ml Falcon tube and close the cap
- Send the sample with air cushion envelop by FEDEX or DHL or equivalent.
Please download and refer to the following documents for information on gDNA methods of preparation and delivery.