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Created page with "<p><span style="color: #000000">A <b>reference genome</b> (also known as a <b>reference assembly</b>) is a digital nucleic acid sequence database, assembled by scientists as a re..."
<p><span style="color: #000000">A <b>reference genome</b> (also known as a <b>reference assembly</b>) is a digital nucleic acid sequence database, assembled by scientists as a representative example of a species' genetic code. As they are often assembled from the sequencing of DNA from a number of donors, reference genomes do not accurately represent the genetic code of any single individual. Instead a reference provides a haploid mosaic of different DNA sequences from each donor. For example <i>GRCh37</i>, the Genome Reference Consortium human genome (build 37) is derived from thirteen anonymous volunteers from Buffalo, New York.<sup id="cite_ref-Editorial_0-0" class="reference"><font size="2">[1]</font></sup><sup id="cite_ref-NYT_1-0" class="reference"><font size="2">[2]</font></sup><sup id="cite_ref-2" class="reference"><font size="2">[3]</font></sup> The ABO blood group system differs among humans, but the human reference genome contains only an O allele (although the other alleles are annotated).<sup id="cite_ref-3" class="reference"><font size="2">[4]</font></sup></span></p>
<p><span style="color: #000000">As the cost of DNA sequencing falls, and new full genome sequencing technologies emerge, more genome sequences continue to be generated. Reference genomes are typically used as a guide on which new genomes are built, enabling them to be assembled much more quickly and cheaply than the initial Human Genome Project. Most individuals with their entire genome sequenced, such as James D. Watson, had their genome assembled in this manner.<sup id="cite_ref-Watson_4-0" class="reference"><font size="2">[5]</font></sup><sup id="cite_ref-5" class="reference"><font size="2">[6]</font></sup> For much of a genome, the reference provides a good approximation of the DNA of any single individual. But in regions with high allelic diversity, such as the major histocompatibility complex in humans and the major urinary proteins of mice, the reference genome may differ significantly from other individuals.<sup id="cite_ref-MHCsc_6-0" class="reference"><font size="2">[7]</font></sup><sup id="cite_ref-Logan_7-0" class="reference"><font size="2">[8]</font></sup><sup id="cite_ref-Hurstchapter_8-0" class="reference"><font size="2">[9]</font></sup> Comparison between the reference (build 36) and Watson's genome revealed 3.3 million single nucleotide polymorphism differences, while about 1.4 percent of his DNA could not be matched to the reference genome at all.<sup id="cite_ref-NYT_1-1" class="reference"><font size="2">[2]</font></sup><sup id="cite_ref-Watson_4-1" class="reference"><font size="2">[5]</font></sup> For regions where there is known to be large scale variation, sets of alternate loci are assembled alongside the reference locus.</span></p>
<p><span style="color: #000000">The human and mouse reference genomes are maintained and improved by the Genome Reference Consortium (GRC), a group of less than 20 scientists from a number of genome research institutes, including the European Bioinformatics Institute, the National Center for Biotechnology Information, The Sanger Institute and Washington University in St. Louis. GRC continues to improve reference genomes by building new alignments that contain fewer gaps, and fixing misrepresentations in the sequence. As of 2010, the human reference genome is in its 19th version. The GRCh37 build contains around 250 gaps, whereas the first version had ~150,000 gaps.<sup id="cite_ref-Editorial_0-1" class="reference"><font size="2">[1]</font></sup></span></p>
<p><span style="color: #000000">Reference genomes can be accessed online at several locations, using dedicated browsers such as Ensembl or UCSC Genome Browser.<sup id="cite_ref-ensembl_9-0" class="reference"><font size="2">[10]</font></sup></span></p>
<h2><span style="color: #000000"><span id="Notes" class="mw-headline">Notes</span></span></h2>
<div class="references-small">
<ol class="references">
<li id="cite_note-Editorial-0">^ <a href="#cite_ref-Editorial_0-0"><sup><i><b><font color="#0645ad" size="2">a</font></b></i></sup></a> <a href="#cite_ref-Editorial_0-1"><sup><i><b><font color="#0645ad" size="2">b</font></b></i></sup></a> <span class="citation Journal">Editorial (October 2010). "E pluribus unum". <i>Nature Methods</i> <b>331</b>: 331. <a title="Digital object identifier" href="/wiki/Digital_object_identifier"><font color="#0645ad">doi</font></a>:<a class="external text" href="http://dx.doi.org/10.1038%2Fnmeth0510-331" rel="nofollow"><font color="#3366bb">10.1038/nmeth0510-331</font></a>.</span><span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=E+pluribus+unum&rft.jtitle=Nature+Methods&rft.aulast=Editorial&rft.au=Editorial&rft.date=October+2010&rft.volume=331&rft.pages=331&rft_id=info:doi/10.1038%2Fnmeth0510-331&rfr_id=info:sid/en.wikipedia.org:Reference_genome"><span style="display: none"> </span></span></li>
<li id="cite_note-NYT-1">^ <a href="#cite_ref-NYT_1-0"><sup><i><b><font color="#0645ad" size="2">a</font></b></i></sup></a> <a href="#cite_ref-NYT_1-1"><sup><i><b><font color="#0645ad" size="2">b</font></b></i></sup></a> <span class="citation news">Wade, Nicholas (May 31, 2007). <a class="external text" href="http://www.nytimes.com/2007/05/31/science/31cnd-gene.html" rel="nofollow"><font color="#3366bb">"Genome of DNA Pioneer Is Deciphered"</font></a>. New York Times<span class="printonly">. <a class="external free" href="http://www.nytimes.com/2007/05/31/science/31cnd-gene.html" rel="nofollow"><font color="#3366bb">http://www.nytimes.com/2007/05/31/science/31cnd-gene.html</font></a></span><span class="reference-accessdate">. Retrieved February 21, 2009</span>.</span><span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook&rft.genre=bookitem&rft.btitle=Genome+of+DNA+Pioneer+Is+Deciphered&rft.atitle=&rft.aulast=Wade&rft.aufirst=Nicholas&rft.au=Wade%2C%26%2332%3BNicholas&rft.date=May+31%2C+2007&rft.pub=New+York+Times&rft_id=http%3A%2F%2Fwww.nytimes.com%2F2007%2F05%2F31%2Fscience%2F31cnd-gene.html&rfr_id=info:sid/en.wikipedia.org:Reference_genome"><span style="display: none"> </span></span></li>
<li id="cite_note-2"><b><a href="#cite_ref-2"><font color="#0645ad">^</font></a></b> Donors were recruited by advertisement in <i><a title="The Buffalo News" href="/wiki/The_Buffalo_News"><font color="#0645ad">The Buffalo News</font></a></i>, on Sunday, March 23, 1997. The first 10 male and 10 female volunteers were invited to make an appointment with the project's <a class="mw-redirect" title="Genetic counselors" href="/wiki/Genetic_counselors"><font color="#0645ad">genetic counselors</font></a> and donate blood from which DNA was extracted. As a result of how the DNA samples were processed, about 80 percent of the reference genome came from eight people and one male individual, designated RP11, accounts for 66 percent of the total.</li>
<li id="cite_note-3"><b><a href="#cite_ref-3"><font color="#0645ad">^</font></a></b> <span class="citation book">Scherer, Stewart (2008). <i>A short guide to the human genome</i>. CSHL Press. p. 135. <a title="International Standard Book Number" href="/wiki/International_Standard_Book_Number"><font color="#0645ad">ISBN</font></a> <a title="Special:BookSources/0879697911" href="/wiki/Special:BookSources/0879697911"><font color="#0645ad">0879697911</font></a>.</span><span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook&rft.genre=book&rft.btitle=A+short+guide+to+the+human+genome&rft.aulast=Scherer&rft.aufirst=Stewart&rft.au=Scherer%2C%26%2332%3BStewart&rft.date=2008&rft.pages=p.%26nbsp%3B135&rft.pub=CSHL+Press&rft.isbn=0879697911&rfr_id=info:sid/en.wikipedia.org:Reference_genome"><span style="display: none"> </span></span></li>
<li id="cite_note-Watson-4">^ <a href="#cite_ref-Watson_4-0"><sup><i><b><font color="#0645ad" size="2">a</font></b></i></sup></a> <a href="#cite_ref-Watson_4-1"><sup><i><b><font color="#0645ad" size="2">b</font></b></i></sup></a> <span class="citation Journal">Wheeler DA, Srinivasan M, Egholm M, Shen Y, Chen L, McGuire A, He W, Chen YJ, Makhijani V, Roth GT, Gomes X, Tartaro K, Niazi F, Turcotte CL, Irzyk GP, Lupski JR, Chinault C, Song XZ, Liu Y, Yuan Y, Nazareth L, Qin X, Muzny DM, Margulies M, Weinstock GM, Gibbs RA, Rothberg JM. (2008). "The complete genome of an individual by massively parallel DNA sequencing". <i>Nature</i> <b>452</b> (7189): 872–6.. <a title="Digital object identifier" href="/wiki/Digital_object_identifier"><font color="#0645ad">doi</font></a>:<a class="external text" href="http://dx.doi.org/10.1038%2Fnature06884" rel="nofollow"><font color="#3366bb">10.1038/nature06884</font></a>. <a class="mw-redirect" title="PubMed Identifier" href="/wiki/PubMed_Identifier"><font color="#0645ad">PMID</font></a> <a class="external text" href="http://www.ncbi.nlm.nih.gov/pubmed/18421352" rel="nofollow"><font color="#3366bb">18421352</font></a>.</span><span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+complete+genome+of+an+individual+by+massively+parallel+DNA+sequencing&rft.jtitle=Nature&rft.aulast=Wheeler+DA%2C+Srinivasan+M%2C+Egholm+M%2C+Shen+Y%2C+Chen+L%2C+McGuire+A%2C+He+W%2C+Chen+YJ%2C+Makhijani+V%2C+Roth+GT%2C+Gomes+X%2C+Tartaro+K%2C+Niazi+F%2C+Turcotte+CL%2C+Irzyk+GP%2C+Lupski+JR%2C+Chinault+C%2C+Song+XZ%2C+Liu+Y%2C+Yuan+Y%2C+Nazareth+L%2C+Qin+X%2C+Muzny+DM%2C+Margulies+M%2C+Weinstock+GM%2C+Gibbs+RA%2C+Rothberg+JM.&rft.au=Wheeler+DA%2C+Srinivasan+M%2C+Egholm+M%2C+Shen+Y%2C+Chen+L%2C+McGuire+A%2C+He+W%2C+Chen+YJ%2C+Makhijani+V%2C+Roth+GT%2C+Gomes+X%2C+Tartaro+K%2C+Niazi+F%2C+Turcotte+CL%2C+Irzyk+GP%2C+Lupski+JR%2C+Chinault+C%2C+Song+XZ%2C+Liu+Y%2C+Yuan+Y%2C+Nazareth+L%2C+Qin+X%2C+Muzny+DM%2C+Margulies+M%2C+Weinstock+GM%2C+Gibbs+RA%2C+Rothberg+JM.&rft.date=2008&rft.volume=452&rft.issue=7189&rft.pages=872%E2%80%936.&rft_id=info:doi/10.1038%2Fnature06884&rft_id=info:pmid/18421352&rfr_id=info:sid/en.wikipedia.org:Reference_genome"><span style="display: none"> </span></span></li>
<li id="cite_note-5"><b><a href="#cite_ref-5"><font color="#0645ad">^</font></a></b> The exception to this is <a class="mw-redirect" title="J. Craig Venter" href="/wiki/J._Craig_Venter"><font color="#0645ad">J. Craig Venter</font></a> whose DNA was sequenced and assembled using <a title="Shotgun sequencing" href="/wiki/Shotgun_sequencing"><font color="#0645ad">shotgun sequencing</font></a> methods.</li>
<li id="cite_note-MHCsc-6"><b><a href="#cite_ref-MHCsc_6-0"><font color="#0645ad">^</font></a></b> <span class="citation Journal">MHC Sequencing Consortium (1999). "Complete sequence and gene map of a human major histocompatibility complex". <i>Nature</i> <b>401</b> (6756): 921–923. <a title="Digital object identifier" href="/wiki/Digital_object_identifier"><font color="#0645ad">doi</font></a>:<a class="external text" href="http://dx.doi.org/10.1038%2F44853" rel="nofollow"><font color="#3366bb">10.1038/44853</font></a>. <a class="mw-redirect" title="PubMed Identifier" href="/wiki/PubMed_Identifier"><font color="#0645ad">PMID</font></a> <a class="external text" href="http://www.ncbi.nlm.nih.gov/pubmed/10553908" rel="nofollow"><font color="#3366bb">10553908</font></a>.</span><span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Complete+sequence+and+gene+map+of+a+human+major+histocompatibility+complex&rft.jtitle=Nature&rft.aulast=MHC+Sequencing+Consortium&rft.au=MHC+Sequencing+Consortium&rft.date=1999&rft.volume=401&rft.issue=6756&rft.pages=921%E2%80%93923&rft_id=info:doi/10.1038%2F44853&rft_id=info:pmid/10553908&rfr_id=info:sid/en.wikipedia.org:Reference_genome"><span style="display: none"> </span></span></li>
<li id="cite_note-Logan-7"><b><a href="#cite_ref-Logan_7-0"><font color="#0645ad">^</font></a></b> <span class="citation Journal">Logan DW, Marton TF, Stowers L (2008). <a class="external text" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2533699" rel="nofollow"><font color="#3366bb">"Species specificity in major urinary proteins by parallel evolution"</font></a>. <i>PLoS ONE</i> <b>3</b> (9): e3280. <a title="Digital object identifier" href="/wiki/Digital_object_identifier"><font color="#0645ad">doi</font></a>:<a class="external text" href="http://dx.doi.org/10.1371%2Fjournal.pone.0003280" rel="nofollow"><font color="#3366bb">10.1371/journal.pone.0003280</font></a>. <a class="mw-redirect" title="PubMed Identifier" href="/wiki/PubMed_Identifier"><font color="#0645ad">PMID</font></a> <a class="external text" href="http://www.ncbi.nlm.nih.gov/pubmed/18815613" rel="nofollow"><font color="#3366bb">18815613</font></a>.</span><span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Species+specificity+in+major+urinary+proteins+by+parallel+evolution&rft.jtitle=PLoS+ONE&rft.aulast=Logan+DW%2C+Marton+TF%2C+Stowers+L&rft.au=Logan+DW%2C+Marton+TF%2C+Stowers+L&rft.date=2008&rft.volume=3&rft.issue=9&rft.pages=e3280&rft_id=info:doi/10.1371%2Fjournal.pone.0003280&rft_id=info:pmid/18815613&rfr_id=info:sid/en.wikipedia.org:Reference_genome"><span style="display: none"> </span></span></li>
<li id="cite_note-Hurstchapter-8"><b><a href="#cite_ref-Hurstchapter_8-0"><font color="#0645ad">^</font></a></b> <span class="citation book">Hurst J, Beynon RJ, Roberts SC, Wyatt TD. (October 2007). <i>Urinary Lipocalins in Rodenta:is there a Generic Model?</i>. Chemical Signals in Vertebrates 11. Springer New York. <a title="International Standard Book Number" href="/wiki/International_Standard_Book_Number"><font color="#0645ad">ISBN</font></a> <a title="Special:BookSources/978-0-387-73944-1" href="/wiki/Special:BookSources/978-0-387-73944-1"><font color="#0645ad">978-0-387-73944-1</font></a>.</span><span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook&rft.genre=book&rft.btitle=Urinary+Lipocalins+in+Rodenta%3Ais+there+a+Generic+Model%3F&rft.aulast=Hurst+J%2C+Beynon+RJ%2C+Roberts+SC%2C+Wyatt+TD.&rft.au=Hurst+J%2C+Beynon+RJ%2C+Roberts+SC%2C+Wyatt+TD.&rft.date=October+2007&rft.series=Chemical+Signals+in+Vertebrates+11&rft.pub=Springer+New+York&rft.isbn=978-0-387-73944-1&rfr_id=info:sid/en.wikipedia.org:Reference_genome"><span style="display: none"> </span></span></li>
<li id="cite_note-ensembl-9"><b><a href="#cite_ref-ensembl_9-0"><font color="#0645ad">^</font></a></b> <span class="citation Journal">Flicek P, Aken BL, Beal K, <i>et al.</i> (January 2008). <a class="external text" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2238821" rel="nofollow"><font color="#3366bb">"Ensembl 2008"</font></a>. <i>Nucleic Acids Res.</i> <b>36</b> (Database issue): D707–14. <a title="Digital object identifier" href="/wiki/Digital_object_identifier"><font color="#0645ad">doi</font></a>:<a class="external text" href="http://dx.doi.org/10.1093%2Fnar%2Fgkm988" rel="nofollow"><font color="#3366bb">10.1093/nar/gkm988</font></a>. <a class="mw-redirect" title="PubMed Identifier" href="/wiki/PubMed_Identifier"><font color="#0645ad">PMID</font></a> <a class="external text" href="http://www.ncbi.nlm.nih.gov/pubmed/18000006" rel="nofollow"><font color="#3366bb">18000006</font></a>.</span><span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Ensembl+2008&rft.jtitle=Nucleic+Acids+Res.&rft.aulast=Flicek+P%2C+Aken+BL%2C+Beal+K%2C+%27%27et+al.%27%27&rft.au=Flicek+P%2C+Aken+BL%2C+Beal+K%2C+%27%27et+al.%27%27&rft.date=January+2008&rft.volume=36&rft.issue=Database+issue&rft.pages=D707%E2%80%9314&rft_id=info:doi/10.1093%2Fnar%2Fgkm988&rft_id=info:pmid/18000006&rfr_id=info:sid/en.wikipedia.org:Reference_genome"><span style="display: none"> </span></span></li>
</ol>
</div>
<h2><span id="External_links" class="mw-headline">External links</span></h2>
<ul>
<li><a class="external text" href="http://www.ncbi.nlm.nih.gov/projects/genome/assembly/grc/" rel="nofollow"><font color="#3366bb">Genome Reference Consortium</font></a></li>
</ul>
<p><span style="color: #000000">As the cost of DNA sequencing falls, and new full genome sequencing technologies emerge, more genome sequences continue to be generated. Reference genomes are typically used as a guide on which new genomes are built, enabling them to be assembled much more quickly and cheaply than the initial Human Genome Project. Most individuals with their entire genome sequenced, such as James D. Watson, had their genome assembled in this manner.<sup id="cite_ref-Watson_4-0" class="reference"><font size="2">[5]</font></sup><sup id="cite_ref-5" class="reference"><font size="2">[6]</font></sup> For much of a genome, the reference provides a good approximation of the DNA of any single individual. But in regions with high allelic diversity, such as the major histocompatibility complex in humans and the major urinary proteins of mice, the reference genome may differ significantly from other individuals.<sup id="cite_ref-MHCsc_6-0" class="reference"><font size="2">[7]</font></sup><sup id="cite_ref-Logan_7-0" class="reference"><font size="2">[8]</font></sup><sup id="cite_ref-Hurstchapter_8-0" class="reference"><font size="2">[9]</font></sup> Comparison between the reference (build 36) and Watson's genome revealed 3.3 million single nucleotide polymorphism differences, while about 1.4 percent of his DNA could not be matched to the reference genome at all.<sup id="cite_ref-NYT_1-1" class="reference"><font size="2">[2]</font></sup><sup id="cite_ref-Watson_4-1" class="reference"><font size="2">[5]</font></sup> For regions where there is known to be large scale variation, sets of alternate loci are assembled alongside the reference locus.</span></p>
<p><span style="color: #000000">The human and mouse reference genomes are maintained and improved by the Genome Reference Consortium (GRC), a group of less than 20 scientists from a number of genome research institutes, including the European Bioinformatics Institute, the National Center for Biotechnology Information, The Sanger Institute and Washington University in St. Louis. GRC continues to improve reference genomes by building new alignments that contain fewer gaps, and fixing misrepresentations in the sequence. As of 2010, the human reference genome is in its 19th version. The GRCh37 build contains around 250 gaps, whereas the first version had ~150,000 gaps.<sup id="cite_ref-Editorial_0-1" class="reference"><font size="2">[1]</font></sup></span></p>
<p><span style="color: #000000">Reference genomes can be accessed online at several locations, using dedicated browsers such as Ensembl or UCSC Genome Browser.<sup id="cite_ref-ensembl_9-0" class="reference"><font size="2">[10]</font></sup></span></p>
<h2><span style="color: #000000"><span id="Notes" class="mw-headline">Notes</span></span></h2>
<div class="references-small">
<ol class="references">
<li id="cite_note-Editorial-0">^ <a href="#cite_ref-Editorial_0-0"><sup><i><b><font color="#0645ad" size="2">a</font></b></i></sup></a> <a href="#cite_ref-Editorial_0-1"><sup><i><b><font color="#0645ad" size="2">b</font></b></i></sup></a> <span class="citation Journal">Editorial (October 2010). "E pluribus unum". <i>Nature Methods</i> <b>331</b>: 331. <a title="Digital object identifier" href="/wiki/Digital_object_identifier"><font color="#0645ad">doi</font></a>:<a class="external text" href="http://dx.doi.org/10.1038%2Fnmeth0510-331" rel="nofollow"><font color="#3366bb">10.1038/nmeth0510-331</font></a>.</span><span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=E+pluribus+unum&rft.jtitle=Nature+Methods&rft.aulast=Editorial&rft.au=Editorial&rft.date=October+2010&rft.volume=331&rft.pages=331&rft_id=info:doi/10.1038%2Fnmeth0510-331&rfr_id=info:sid/en.wikipedia.org:Reference_genome"><span style="display: none"> </span></span></li>
<li id="cite_note-NYT-1">^ <a href="#cite_ref-NYT_1-0"><sup><i><b><font color="#0645ad" size="2">a</font></b></i></sup></a> <a href="#cite_ref-NYT_1-1"><sup><i><b><font color="#0645ad" size="2">b</font></b></i></sup></a> <span class="citation news">Wade, Nicholas (May 31, 2007). <a class="external text" href="http://www.nytimes.com/2007/05/31/science/31cnd-gene.html" rel="nofollow"><font color="#3366bb">"Genome of DNA Pioneer Is Deciphered"</font></a>. New York Times<span class="printonly">. <a class="external free" href="http://www.nytimes.com/2007/05/31/science/31cnd-gene.html" rel="nofollow"><font color="#3366bb">http://www.nytimes.com/2007/05/31/science/31cnd-gene.html</font></a></span><span class="reference-accessdate">. Retrieved February 21, 2009</span>.</span><span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook&rft.genre=bookitem&rft.btitle=Genome+of+DNA+Pioneer+Is+Deciphered&rft.atitle=&rft.aulast=Wade&rft.aufirst=Nicholas&rft.au=Wade%2C%26%2332%3BNicholas&rft.date=May+31%2C+2007&rft.pub=New+York+Times&rft_id=http%3A%2F%2Fwww.nytimes.com%2F2007%2F05%2F31%2Fscience%2F31cnd-gene.html&rfr_id=info:sid/en.wikipedia.org:Reference_genome"><span style="display: none"> </span></span></li>
<li id="cite_note-2"><b><a href="#cite_ref-2"><font color="#0645ad">^</font></a></b> Donors were recruited by advertisement in <i><a title="The Buffalo News" href="/wiki/The_Buffalo_News"><font color="#0645ad">The Buffalo News</font></a></i>, on Sunday, March 23, 1997. The first 10 male and 10 female volunteers were invited to make an appointment with the project's <a class="mw-redirect" title="Genetic counselors" href="/wiki/Genetic_counselors"><font color="#0645ad">genetic counselors</font></a> and donate blood from which DNA was extracted. As a result of how the DNA samples were processed, about 80 percent of the reference genome came from eight people and one male individual, designated RP11, accounts for 66 percent of the total.</li>
<li id="cite_note-3"><b><a href="#cite_ref-3"><font color="#0645ad">^</font></a></b> <span class="citation book">Scherer, Stewart (2008). <i>A short guide to the human genome</i>. CSHL Press. p. 135. <a title="International Standard Book Number" href="/wiki/International_Standard_Book_Number"><font color="#0645ad">ISBN</font></a> <a title="Special:BookSources/0879697911" href="/wiki/Special:BookSources/0879697911"><font color="#0645ad">0879697911</font></a>.</span><span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook&rft.genre=book&rft.btitle=A+short+guide+to+the+human+genome&rft.aulast=Scherer&rft.aufirst=Stewart&rft.au=Scherer%2C%26%2332%3BStewart&rft.date=2008&rft.pages=p.%26nbsp%3B135&rft.pub=CSHL+Press&rft.isbn=0879697911&rfr_id=info:sid/en.wikipedia.org:Reference_genome"><span style="display: none"> </span></span></li>
<li id="cite_note-Watson-4">^ <a href="#cite_ref-Watson_4-0"><sup><i><b><font color="#0645ad" size="2">a</font></b></i></sup></a> <a href="#cite_ref-Watson_4-1"><sup><i><b><font color="#0645ad" size="2">b</font></b></i></sup></a> <span class="citation Journal">Wheeler DA, Srinivasan M, Egholm M, Shen Y, Chen L, McGuire A, He W, Chen YJ, Makhijani V, Roth GT, Gomes X, Tartaro K, Niazi F, Turcotte CL, Irzyk GP, Lupski JR, Chinault C, Song XZ, Liu Y, Yuan Y, Nazareth L, Qin X, Muzny DM, Margulies M, Weinstock GM, Gibbs RA, Rothberg JM. (2008). "The complete genome of an individual by massively parallel DNA sequencing". <i>Nature</i> <b>452</b> (7189): 872–6.. <a title="Digital object identifier" href="/wiki/Digital_object_identifier"><font color="#0645ad">doi</font></a>:<a class="external text" href="http://dx.doi.org/10.1038%2Fnature06884" rel="nofollow"><font color="#3366bb">10.1038/nature06884</font></a>. <a class="mw-redirect" title="PubMed Identifier" href="/wiki/PubMed_Identifier"><font color="#0645ad">PMID</font></a> <a class="external text" href="http://www.ncbi.nlm.nih.gov/pubmed/18421352" rel="nofollow"><font color="#3366bb">18421352</font></a>.</span><span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+complete+genome+of+an+individual+by+massively+parallel+DNA+sequencing&rft.jtitle=Nature&rft.aulast=Wheeler+DA%2C+Srinivasan+M%2C+Egholm+M%2C+Shen+Y%2C+Chen+L%2C+McGuire+A%2C+He+W%2C+Chen+YJ%2C+Makhijani+V%2C+Roth+GT%2C+Gomes+X%2C+Tartaro+K%2C+Niazi+F%2C+Turcotte+CL%2C+Irzyk+GP%2C+Lupski+JR%2C+Chinault+C%2C+Song+XZ%2C+Liu+Y%2C+Yuan+Y%2C+Nazareth+L%2C+Qin+X%2C+Muzny+DM%2C+Margulies+M%2C+Weinstock+GM%2C+Gibbs+RA%2C+Rothberg+JM.&rft.au=Wheeler+DA%2C+Srinivasan+M%2C+Egholm+M%2C+Shen+Y%2C+Chen+L%2C+McGuire+A%2C+He+W%2C+Chen+YJ%2C+Makhijani+V%2C+Roth+GT%2C+Gomes+X%2C+Tartaro+K%2C+Niazi+F%2C+Turcotte+CL%2C+Irzyk+GP%2C+Lupski+JR%2C+Chinault+C%2C+Song+XZ%2C+Liu+Y%2C+Yuan+Y%2C+Nazareth+L%2C+Qin+X%2C+Muzny+DM%2C+Margulies+M%2C+Weinstock+GM%2C+Gibbs+RA%2C+Rothberg+JM.&rft.date=2008&rft.volume=452&rft.issue=7189&rft.pages=872%E2%80%936.&rft_id=info:doi/10.1038%2Fnature06884&rft_id=info:pmid/18421352&rfr_id=info:sid/en.wikipedia.org:Reference_genome"><span style="display: none"> </span></span></li>
<li id="cite_note-5"><b><a href="#cite_ref-5"><font color="#0645ad">^</font></a></b> The exception to this is <a class="mw-redirect" title="J. Craig Venter" href="/wiki/J._Craig_Venter"><font color="#0645ad">J. Craig Venter</font></a> whose DNA was sequenced and assembled using <a title="Shotgun sequencing" href="/wiki/Shotgun_sequencing"><font color="#0645ad">shotgun sequencing</font></a> methods.</li>
<li id="cite_note-MHCsc-6"><b><a href="#cite_ref-MHCsc_6-0"><font color="#0645ad">^</font></a></b> <span class="citation Journal">MHC Sequencing Consortium (1999). "Complete sequence and gene map of a human major histocompatibility complex". <i>Nature</i> <b>401</b> (6756): 921–923. <a title="Digital object identifier" href="/wiki/Digital_object_identifier"><font color="#0645ad">doi</font></a>:<a class="external text" href="http://dx.doi.org/10.1038%2F44853" rel="nofollow"><font color="#3366bb">10.1038/44853</font></a>. <a class="mw-redirect" title="PubMed Identifier" href="/wiki/PubMed_Identifier"><font color="#0645ad">PMID</font></a> <a class="external text" href="http://www.ncbi.nlm.nih.gov/pubmed/10553908" rel="nofollow"><font color="#3366bb">10553908</font></a>.</span><span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Complete+sequence+and+gene+map+of+a+human+major+histocompatibility+complex&rft.jtitle=Nature&rft.aulast=MHC+Sequencing+Consortium&rft.au=MHC+Sequencing+Consortium&rft.date=1999&rft.volume=401&rft.issue=6756&rft.pages=921%E2%80%93923&rft_id=info:doi/10.1038%2F44853&rft_id=info:pmid/10553908&rfr_id=info:sid/en.wikipedia.org:Reference_genome"><span style="display: none"> </span></span></li>
<li id="cite_note-Logan-7"><b><a href="#cite_ref-Logan_7-0"><font color="#0645ad">^</font></a></b> <span class="citation Journal">Logan DW, Marton TF, Stowers L (2008). <a class="external text" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2533699" rel="nofollow"><font color="#3366bb">"Species specificity in major urinary proteins by parallel evolution"</font></a>. <i>PLoS ONE</i> <b>3</b> (9): e3280. <a title="Digital object identifier" href="/wiki/Digital_object_identifier"><font color="#0645ad">doi</font></a>:<a class="external text" href="http://dx.doi.org/10.1371%2Fjournal.pone.0003280" rel="nofollow"><font color="#3366bb">10.1371/journal.pone.0003280</font></a>. <a class="mw-redirect" title="PubMed Identifier" href="/wiki/PubMed_Identifier"><font color="#0645ad">PMID</font></a> <a class="external text" href="http://www.ncbi.nlm.nih.gov/pubmed/18815613" rel="nofollow"><font color="#3366bb">18815613</font></a>.</span><span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Species+specificity+in+major+urinary+proteins+by+parallel+evolution&rft.jtitle=PLoS+ONE&rft.aulast=Logan+DW%2C+Marton+TF%2C+Stowers+L&rft.au=Logan+DW%2C+Marton+TF%2C+Stowers+L&rft.date=2008&rft.volume=3&rft.issue=9&rft.pages=e3280&rft_id=info:doi/10.1371%2Fjournal.pone.0003280&rft_id=info:pmid/18815613&rfr_id=info:sid/en.wikipedia.org:Reference_genome"><span style="display: none"> </span></span></li>
<li id="cite_note-Hurstchapter-8"><b><a href="#cite_ref-Hurstchapter_8-0"><font color="#0645ad">^</font></a></b> <span class="citation book">Hurst J, Beynon RJ, Roberts SC, Wyatt TD. (October 2007). <i>Urinary Lipocalins in Rodenta:is there a Generic Model?</i>. Chemical Signals in Vertebrates 11. Springer New York. <a title="International Standard Book Number" href="/wiki/International_Standard_Book_Number"><font color="#0645ad">ISBN</font></a> <a title="Special:BookSources/978-0-387-73944-1" href="/wiki/Special:BookSources/978-0-387-73944-1"><font color="#0645ad">978-0-387-73944-1</font></a>.</span><span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook&rft.genre=book&rft.btitle=Urinary+Lipocalins+in+Rodenta%3Ais+there+a+Generic+Model%3F&rft.aulast=Hurst+J%2C+Beynon+RJ%2C+Roberts+SC%2C+Wyatt+TD.&rft.au=Hurst+J%2C+Beynon+RJ%2C+Roberts+SC%2C+Wyatt+TD.&rft.date=October+2007&rft.series=Chemical+Signals+in+Vertebrates+11&rft.pub=Springer+New+York&rft.isbn=978-0-387-73944-1&rfr_id=info:sid/en.wikipedia.org:Reference_genome"><span style="display: none"> </span></span></li>
<li id="cite_note-ensembl-9"><b><a href="#cite_ref-ensembl_9-0"><font color="#0645ad">^</font></a></b> <span class="citation Journal">Flicek P, Aken BL, Beal K, <i>et al.</i> (January 2008). <a class="external text" href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2238821" rel="nofollow"><font color="#3366bb">"Ensembl 2008"</font></a>. <i>Nucleic Acids Res.</i> <b>36</b> (Database issue): D707–14. <a title="Digital object identifier" href="/wiki/Digital_object_identifier"><font color="#0645ad">doi</font></a>:<a class="external text" href="http://dx.doi.org/10.1093%2Fnar%2Fgkm988" rel="nofollow"><font color="#3366bb">10.1093/nar/gkm988</font></a>. <a class="mw-redirect" title="PubMed Identifier" href="/wiki/PubMed_Identifier"><font color="#0645ad">PMID</font></a> <a class="external text" href="http://www.ncbi.nlm.nih.gov/pubmed/18000006" rel="nofollow"><font color="#3366bb">18000006</font></a>.</span><span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Ensembl+2008&rft.jtitle=Nucleic+Acids+Res.&rft.aulast=Flicek+P%2C+Aken+BL%2C+Beal+K%2C+%27%27et+al.%27%27&rft.au=Flicek+P%2C+Aken+BL%2C+Beal+K%2C+%27%27et+al.%27%27&rft.date=January+2008&rft.volume=36&rft.issue=Database+issue&rft.pages=D707%E2%80%9314&rft_id=info:doi/10.1093%2Fnar%2Fgkm988&rft_id=info:pmid/18000006&rfr_id=info:sid/en.wikipedia.org:Reference_genome"><span style="display: none"> </span></span></li>
</ol>
</div>
<h2><span id="External_links" class="mw-headline">External links</span></h2>
<ul>
<li><a class="external text" href="http://www.ncbi.nlm.nih.gov/projects/genome/assembly/grc/" rel="nofollow"><font color="#3366bb">Genome Reference Consortium</font></a></li>
</ul>
