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Created page with "<h1><span id="1..C2.A0Sample.28gDNA.29_Preparation" class="mw-headline">1. Sample(gDNA) Preparation</span></h1> <p> </p> <p>1) DNA should be dissolved in TE ..."
<h1><span id="1..C2.A0Sample.28gDNA.29_Preparation" class="mw-headline">1. Sample(gDNA) Preparation</span></h1>
<p> </p>
<p>1) DNA should be dissolved in TE buffer (Tris 10mM pH 8,0, 0.1 mM EDTA) or 10 mM Tris pH 8.0.</p>
<p>2) Do Not Dissolve in Water</p>
<p> </p>
<p> </p>
<h1><span id="2..C2.A0Sample.28gDNA.29_Requirement" class="mw-headline">2. Sample(gDNA) Requirement</span></h1>
<p> </p>
<p>1) Amount and concentration spec. : over 20 ug of gDNA with >50 ng/ul</p>
<p> This amount is required for</p>
<p>a. Whole genome sequencing (30X)</p>
<p>b. Whole exome sequencing (50X)</p>
<p>c. Genotyping</p>
<p>2) Sample(gDNA) should be : OD260/280=1.8~2.0; free of protein, RNA, or other visible contamination</p>
<p> </p>
<p> </p>
<h1><span id="3..C2.A0Sample.28gDNA.29_Packing_Information.C2.A0" class="mw-headline">3. Sample(gDNA) Packing Information </span></h1>
<p> </p>
<p>This way is only available when DNA is dissolved in TE or Tris buffer.</p>
<p>1) If not, please pack DNA with icepack in icebox and use Fedex or DHL service</p>
<p>2) Mark sample information on tube (concentration, sample name…what you want)</p>
<p>3) Seal the cap of tube with parafilm well</p>
<p>4) Wrap the tube with paper towel or equivalent</p>
<p>5) Put into the 50 ml Falcon tube and close the cap</p>
<p>6) Send the sample with air cushion envelop by FEDEX or DHL or equivalent</p>
<p> </p>
<p> </p>
<h1><span id="4..C2.A0Requirement_of_Sample.28gDNA.29_Information" class="mw-headline">4. Requirement of Sample(gDNA) Information</span></h1>
<p> </p>
<p>1) Sample type: DNA</p>
<p>2) Condition: DNA in TE buffer or EB buffer or equivalent. Not in water</p>
<p>3) Gel image of DNA electrophoresis</p>
<p>4) Species</p>
<p>5) No. of Tubes</p>
<p>6) Concentration (ng/μl)</p>
<p>7) Volume (μl)</p>
<p>8) Total Quantity (μg)</p>
<p>9) OD260/280</p>
<p>10) OD260/230</p>
<p> </p>
<p> </p>
<p> </p>
<p><b><span style="font-size: medium">Please download and refer to the following documents for information on gDNA methods of preparation and delivery.</span></b></p>
<p> </p>
<p>1.<span style="color: #800000"><a href="/userfiles/Method of Sample(gDNA) Preparation for Sending out.doc">Method of Sample (gDNA) Preparation for Sending out</a></span></p>
<p>2.<span style="color: #800000"><a href="/userfiles/Declaration_of_Shipment_for_anyone(1).doc">Declaration of Shipment</a><br />
</span></p>
<p> </p>
<p>1) DNA should be dissolved in TE buffer (Tris 10mM pH 8,0, 0.1 mM EDTA) or 10 mM Tris pH 8.0.</p>
<p>2) Do Not Dissolve in Water</p>
<p> </p>
<p> </p>
<h1><span id="2..C2.A0Sample.28gDNA.29_Requirement" class="mw-headline">2. Sample(gDNA) Requirement</span></h1>
<p> </p>
<p>1) Amount and concentration spec. : over 20 ug of gDNA with >50 ng/ul</p>
<p> This amount is required for</p>
<p>a. Whole genome sequencing (30X)</p>
<p>b. Whole exome sequencing (50X)</p>
<p>c. Genotyping</p>
<p>2) Sample(gDNA) should be : OD260/280=1.8~2.0; free of protein, RNA, or other visible contamination</p>
<p> </p>
<p> </p>
<h1><span id="3..C2.A0Sample.28gDNA.29_Packing_Information.C2.A0" class="mw-headline">3. Sample(gDNA) Packing Information </span></h1>
<p> </p>
<p>This way is only available when DNA is dissolved in TE or Tris buffer.</p>
<p>1) If not, please pack DNA with icepack in icebox and use Fedex or DHL service</p>
<p>2) Mark sample information on tube (concentration, sample name…what you want)</p>
<p>3) Seal the cap of tube with parafilm well</p>
<p>4) Wrap the tube with paper towel or equivalent</p>
<p>5) Put into the 50 ml Falcon tube and close the cap</p>
<p>6) Send the sample with air cushion envelop by FEDEX or DHL or equivalent</p>
<p> </p>
<p> </p>
<h1><span id="4..C2.A0Requirement_of_Sample.28gDNA.29_Information" class="mw-headline">4. Requirement of Sample(gDNA) Information</span></h1>
<p> </p>
<p>1) Sample type: DNA</p>
<p>2) Condition: DNA in TE buffer or EB buffer or equivalent. Not in water</p>
<p>3) Gel image of DNA electrophoresis</p>
<p>4) Species</p>
<p>5) No. of Tubes</p>
<p>6) Concentration (ng/μl)</p>
<p>7) Volume (μl)</p>
<p>8) Total Quantity (μg)</p>
<p>9) OD260/280</p>
<p>10) OD260/230</p>
<p> </p>
<p> </p>
<p> </p>
<p><b><span style="font-size: medium">Please download and refer to the following documents for information on gDNA methods of preparation and delivery.</span></b></p>
<p> </p>
<p>1.<span style="color: #800000"><a href="/userfiles/Method of Sample(gDNA) Preparation for Sending out.doc">Method of Sample (gDNA) Preparation for Sending out</a></span></p>
<p>2.<span style="color: #800000"><a href="/userfiles/Declaration_of_Shipment_for_anyone(1).doc">Declaration of Shipment</a><br />
</span></p>
