<strong>Polony DNA sequencing.</strong><br /><br /><br />Porreca GJ, Shendure J, Church GM.<br /><br /><span titlestyle="Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]."><a href="javascriptfont-size:AL_get(this, 'jour', 'Curr Protoc Mol Biol.')16px;">Curr Protoc Mol Biol.</a></span> 2006 Nov;Chapter 7:Unit 7.8.<br /><br /><p class="affiliation">Harvard Medical School, Boston, Massachusetts, USA.</p><p class="abstract">Polony DNA sequencing provides an inexpensive, accurate, high-throughput way to resequence genomes of interest by comparison to a reference genome. Mate-paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR. These serve as templates for sequencing by fluorescent nonamer ligation reactions on a microscope slide. Each sequencing run results in millions of 26-bp reads that can be aligned to the reference genome, allowing the identification of differences between sequences.<br/p><p class="pmid"br/>PMID[https: 18265387 [PubMed - indexed for MEDLINE//currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1002/0471142727.mb0708s76 Link to full article]<br /><br /><hr br/> Porreca GJ, Shendure J, Church GM. Harvard Medical School, Boston, Massachusetts, USA.<br/><br /><span title="Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.].">[[Curr Protoc Mol Biol.]]</span> 2006.11.</pspan>
