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<strong>Polony DNA sequencing.</strong><br />
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Porreca GJ, Shendure J, Church GM.<br />
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<span title="Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]."><a href="javascript:AL_get(this, 'jour', 'Curr Protoc Mol Biol.');">Curr Protoc Mol Biol.</a></span> 2006 Nov;Chapter 7:Unit 7.8.<br />
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<p class="affiliation">Harvard Medical School, Boston, Massachusetts, USA.</p>
<p class="abstract">Polony DNA sequencing provides an inexpensive, accurate, high-throughput way to resequence genomes of interest by comparison to a reference genome. Mate-paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR. These serve as templates for sequencing by fluorescent nonamer ligation reactions on a microscope slide. Each sequencing run results in millions of 26-bp reads that can be aligned to the reference genome, allowing the identification of differences between sequences.</p>
<p class="pmid">PMID: 18265387 [PubMed - indexed for MEDLINE]<br />
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</p>
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Porreca GJ, Shendure J, Church GM.<br />
<br />
<span title="Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]."><a href="javascript:AL_get(this, 'jour', 'Curr Protoc Mol Biol.');">Curr Protoc Mol Biol.</a></span> 2006 Nov;Chapter 7:Unit 7.8.<br />
<br />
<p class="affiliation">Harvard Medical School, Boston, Massachusetts, USA.</p>
<p class="abstract">Polony DNA sequencing provides an inexpensive, accurate, high-throughput way to resequence genomes of interest by comparison to a reference genome. Mate-paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR. These serve as templates for sequencing by fluorescent nonamer ligation reactions on a microscope slide. Each sequencing run results in millions of 26-bp reads that can be aligned to the reference genome, allowing the identification of differences between sequences.</p>
<p class="pmid">PMID: 18265387 [PubMed - indexed for MEDLINE]<br />
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